Yewubnesh Wendimu Seifu
Brno
Summary
A Plant Molecular Biologist with more than five years experience of researching in Plant Cell and Developmental Biology demonstrated with publication records. Acquired a solid experience in supervising students and teaching plant physiology, and post-harvest physiology courses. Demonstrates strong experiences in various molecular biology techniques, a very good English communication and organizational skills, and proven capability of working independently and in collaboration with culturally diverse teams. Aspires to work as a post-doctoral researcher in the field of plant biology research.
Overview
11
11
years of professional experience
Work History
Research Specialist
Central European Institute of Technology (CEITEC)
10.2017 - 03.2022
- Demonstrated the link between the structure and functional activity of auxin transporter PIN5 and PIN8 proteins, and indicated that these proteins have divergent topology and antagonistic activities in root development.
Director of Gender Directorate
Arsi University
08.2016 - 09.2017
- Improved participation of women academic staff in research and community services.
- Provided life skill training for more than 300 first year undergraduate students.
- Served as Arsi University Senate Academic committee.
Lecturer and Researcher
Arsi University
10.2014 - 09.2017
- Taught Post-harvest Technology and Plant Physiology courses delivered to more than one hundred 3rd year undergraduate students.
- Evaluated students’ progress through different assessment techniques, and provided a detail feedback to support their professional development.
- Supervised total of ten 3rd year Plant Sciences students and provide them an excellent guide during their thesis work.
- Secured a research grant for two years, conducted a study on potato late blight and published an article from the project.
Lecturer
Ambo University
09.2013 - 08.2014
- Taught Plant Physiology and Post-Harvest Technology courses offered to more than Seventy-five 3rd year students.
- Evaluated students’ progress through different assessment techniques, and provided a detailed feedback to support their professional development.
Research Assistant
Wageningen University
09.2012 - 08.2013
- Analyzed glucose, fructose, and sucrose content in lettuce using HPLC.
- Co-authored two scientific articles.
- Studied an interaction between Rhamphicarpa fistulosa and rice (https://edepot.wur.nl/276724).
Graduate Assistant
Ambo University
09.2010 - 08.2011
- Assisted teaching Plant Physiology course provided to 65 undergraduate students.
Education
PhD - Life Sciences
Masaryk University
Brno, Czech Republic.01.2025
MSc. - Plant Sciences
Wageningen University
Wageningen, The Netherlands. 08.2013
BSc. - Crop Production
Ambo University
Ambo, Ethiopia.06.2010
Skills
- Hands on experience with different molecular biology skills such as:
- Molecular cloning techniques (Gateway, TOPO-TA, and restriction digestion)
- DNA and RNA extraction, PCR, Gel electrophoresis
- Transient gene expression in Nicotiana tabacum leaves, Arabidopsis thaliana transformation for stable protein expression,
- Crossing Arabidopsis, and T-DNA genotyping
- Immunocytochemistry
- Escherichia coli, and Agrobacterium tumefaciens transformation
- Confocal Microscopy (LSM 700 and 780 systems) and AxioScope
- HPLC, GC
- Proficient in bioinformatics skills such as online databases (eg AlphaFold, NCBI, EMBL-EBI, TAIR), PyMOL (to visualization and analysis protein structure), BioEdit Sequence Alignment Editor, R-studio and Genstat (for statistical data analysis), Image J, and Inkscape, and intermediate skill in RNA-seq data analysis
- Advanced experience in other research and technical skills:
- Excellent communication level (Advanced English language, scientific presentations and teaching skills, and A1 level in Deutsch (German language))
- Advanced skills in designing field and greenhouse pot experiments based on the appropriate experimental design (eg RCBD, Split-Plot Design)
- Solid knowledge of root cell and developmental biology
- Highly organized, detail-oriented, problem-solving and solution-oriented mindset
- Excellent team player and demonstrated a strong ability to work independently and take responsibility
- Experience of working with potato, rice and Arabidopsis
- An expert in plant physiology, and post-harvest physiology of horticultural products
Awards
10/2017 – 3/2022: Czech Ministry of Education Scholarship, Czech Republic., 1/2015 – 9/2017: Potato Research Grant, Arsi University, Ethiopia., 9/2011 – 8/2013: Netherlands Fellowship Program (NUFFIC) scholarship, Netherlands.
Publications (my name is indicated in bold)
- Seifu, Y.W., Pukyšová, V., Rýdza, N. et al. Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology. Plant Methods 20, 84 (2024). https://doi.org/10.1186/s13007-024-01182-7
- Zwiewka M, Bilaniovčová V, Seifu Y.W and Nodzyński T (2019). The Nuts and Bolts of PIN Auxin Efflux Carriers. Front. Plant Sci. 10:985. DOI: https://doi.org/10.3389/fpls.2019.00985
- Seifu, Y.W. Reducing Severity of Late Blight (Phytophthora infestans) and Improving Potato (Solanum tuberosum L.) Tuber Yield with Pre-Harvest Application of Calcium Nutrients. Agronomy 2017, 7, 69. DOI: https://www.mdpi.com/2073-4395/7/4/69
- Woltering, E.J., and Seifu, Y.W. (2015). Low intensity monochromatic red, blue, or green light increases the carbohydrate levels and substantially extends the shelf life of fresh-cut lettuce. Acta Hortic. 1079, 257-264. https://www.actahort.org/books/1079/1079_30.htm
- Woltering, E.J., Wang, J.-F. and Seifu, Y.W. (2016). Storage of fresh-cut lettuce at light levels below the light compensation point increases sugar levels and greatly prolongs the shelf-life. Acta Hortic. 1141, 357-364. https://doi.org/10.17660/ActaHortic.2016.1141.45
CONFERENCE AND SEMINARS
Topic of presentation: Topology of Endoplasmic Reticulum PIN proteins: PIN5 vs PIN8 “the Heads and Tails of Auxin Flux?”.
20-23/9/2021: Oral Presentation, International Experimental Plant Biology Conference. Virtually taking
place in Katowice, Poland.
08/10/2019: Oral Presentation at Mendel Centre Seminar. Brno, Czech Republic.
27-30/8/2019: Poster Presentation, Plant Biology conference. České Budějovice, Czech Republic.
Timeline
Research Specialist
Central European Institute of Technology (CEITEC)
10.2017 - 03.2022
Director of Gender Directorate
Arsi University
08.2016 - 09.2017
Lecturer and Researcher
Arsi University
10.2014 - 09.2017
Lecturer
Ambo University
09.2013 - 08.2014
Research Assistant
Wageningen University
09.2012 - 08.2013
Graduate Assistant
Ambo University
09.2010 - 08.2011
PhD - Life Sciences
Masaryk University
MSc. - Plant Sciences
Wageningen University
BSc. - Crop Production
Ambo University
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