Co-trainer for Visiting Scientist Dr. Saurav Kumar from CIFE, Mumbai, India. Trained the scientist on different types of comet assay and embryotoxicity.
Reviewer
Reviewed 3 manuscripts in the following journals: Open Veterinary Journal, Environmental Pollution, Biomedicine & Pharmacotherapy.
PhD Consultant
01.2024 - Current
PhD Consultant for PhD student Mohammad Rashed, MSc.
Summer School Project Investigator
University of Valencia
06.2023 - 07.2023
Summer School Project Investigator for a Master’s Student from the University of Valencia, Spain (Alvaro González-Cid).
Education
PhD - Fisheries, Molecular Biology
University of South Bohemia
Ceske Budejovice, Czech Republic
09.2025
Master of Fisheries Science - Aquatic Environment Management Division
Central Institute of Fisheries Education, ICAR
Mumbai
11.2020
Bachelor of Fisheries Science - Fisheries Science
College of Fisheries, Central Agricultural University
Tripura
07.2018
Skills
Experience in handling live animals, culturing sterlet and zebrafish embryos, western blotting, Immunohistochemistry, In situ hybridization, histology, confocal microscopy, qPCR, and comet assay, SDS PAGE, DNA electrophoresis
Ievgeniia, Gazo, Research Scientist, gazo@frov.jcu.cz, Laboratory of Cellular, Molecular and Quantitative Genetics, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Czech Republic
Accomplishments
Underwent a fully funded Ph.D. program from the Ministry of Education, Youth and Sports, Czech Republic.
Won the Best Student Presentation Award three times at the Ph.D. student seminar at the Faculty of Fisheries and Protection of Water in 2023, 2024, and 2025.
2024 – Travel grant from the Society of European Comparative Endocrinologists to attend the 31st Conference on European Comparative Endocrinologists, Italy, 2024.
2023 – Travel grant ERASMUS for 3-month internship at Warsaw University of Life Sciences.
2020 - Dr. Hiralal Chaudhuri Award in Post Graduate Program, Department of Aquatic Environment and Health Management. ICAR-CIFE, Mumbai, India.
2018 - 9th in Best Fisheries Graduate of India Exam under Professional Fisheries Graduate Forum.
2018 - Gold Medal for Standing First in Undergraduate Program.
2018 - Ranked Sixth in ICAR entrance examination for master's degree program; Erstwhile JRF Fellowship
Publications
Dey, A., Nayak, R., Prchal, M., Gonzalez-Cid, A., Pšenicka, M., Šindelka, R., Flajšhans, M., Gazo, I., 2024. Species-specific differences in DNA damage sensitivity at early developmental stages: A comparative study of sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio). Environ. Toxicol. Pharmacol., 110, 104501 https://doi.org/10.1016/j.etap.2024.104501
Gazo, I., Dey, A., Franěk, R., Kahanece Güralp, H., Lebeda, I., Flajšhans, M., Pšenicka, M., 2024. Inhibition of Atm and Atr kinases affects embryo development and the DNA damage response in sterlet (Acipenser ruthenus): aquaculture, 590, 741146, https://doi.org/10.1016/j.aquaculture.2024.741146.
Dey, A., Flajšhans, M., Pšenicka, M., Gazo, I., 2023. DNA repair genes play a variety of roles in the development of fish embryos. Front. Cell Dev. Biol., 11, 1119229. https://doi.org/10.3389/fcell.2023.1119229.
Dey, A., Rzepkowska, M., Fajkowska, M., Steinbach, C., Pšenicka, M., Šindelka, Franek R., Flajšhans, M., Gazo, I., 2024. Effect of methyl methanesulfonate on the developmental role of AP endonuclease (APEX1) in sterlet (Acipenser ruthenus) embryos. (in preparation).
Dey, A., Rzepkowska, M., Fajkowska, M., Steinbach, C., Pšenicka, M., Šindelka, Franek R., Flajšhans, M., Gazo, I., 2024. Comparative Assessment of Fixatives and Tissue Processing Protocols on Sterlet (Acipenser ruthenus) Embryo. (in preparation).
Training
Training in Immunohistochemistry of zebrafish embryos, Department of Ichthyology and Biotechnology in Aquaculture, Warsaw University of Life Science, Poland, 1.8.2023 – 11.11.2023. Recommendation: Malgorzata Rzepkowska, PhD, malgorzata_rzepkowska@sggw.edu.pl.
Training in qPCR, Laboratory of Gene Expression, Institute of Biotechnology, Czech Academy of Science, 18.12.2021 – 21.12.2021. Recommendation: Šindelka Radek, Ph.D, Radek.Sindelka@ibt.cas.cz.
Ph.D. Thesis Description
Embryos of oviparous aquatic organisms are continuously exposed to water pollutants. A wide range of xenobiotics can damage the DNA of developing embryos, disturbing embryogenesis. My PhD. research work has evidenced that fish embryos have evolved a developmental stage-specific DNA repair machinery to combat DNA damage. This information is crucial for the ecological risk assessment of fish embryos. Additionally, several studies have reported the indispensable role of DNA repair proteins in zebrafish organogenesis using gene-editing approaches. However, under natural conditions, there can be either an increase or a decrease in protein expression in response to stress. Thus, we aimed to investigate how exogenous DNA damage during embryogenesis can disturb the basal expression of DNA repair proteins and how this disturbance impairs their developmental role. This can explain one of the reasons for embryonic malformations in response to genotoxic stress. We also characterized the species-specific DNA damage response during fish embryo development and identified that early-stage embryos are more sensitive to DNA damage than later stages. During my Ph.D., I worked on the evolutionarily conserved non-model species sterlet (Acipenser ruthenus), common carp (Cyprinus carpio), and zebrafish (Danio rerio). We chose two evolutionarily distant fish species to delineate the evolutionary conservation of DNA repair machinery during fish embryo development.
Timeline
PhD Consultant
01.2024 - Current
Summer School Project Investigator
University of Valencia
06.2023 - 07.2023
Co-trainer for Visiting Scientist
CIFE, Mumbai, India
02.2023 - 02.2023
Reviewer
PhD - Fisheries, Molecular Biology
University of South Bohemia
Master of Fisheries Science - Aquatic Environment Management Division
Central Institute of Fisheries Education, ICAR
Bachelor of Fisheries Science - Fisheries Science
College of Fisheries, Central Agricultural University
Facility Manager at Jones Lang Lasalle Property Consultants India Pvt. Ltd. (Deployed at Facebook India Online Services Pvt. Ltd. Mumbai as Facility Manager)Facility Manager at Jones Lang Lasalle Property Consultants India Pvt. Ltd. (Deployed at Facebook India Online Services Pvt. Ltd. Mumbai as Facility Manager)